Application note for Clomipramine

✅Clomipramine can be ionized and is therefore suitable for EME.

About Clomipramine

Clomipramine is a dibenzoazepine that is 10,11-dihydro-5H-dibenzo[b,f]azepine which is substituted by chlorine at position 3 and in which the hydrogen attached to the nitrogen is replaced by a 3-(dimethylamino)propyl group. One of the more sedating tricyclic antidepressants, it is used as the hydrochloride salt for the treatment of depression as well as obsessive-compulsive disorder and phobias. It has a role as a serotonergic antagonist, a serotonergic drug, a serotonin uptake inhibitor, an EC 1.8.1.12 (trypanothione-disulfide reductase) inhibitor, an antidepressant and an anticoronaviral agent. It is functionally related to an imipramine. It is a conjugate base of a clomipramine(1+).

What is Electromembrane Extraction (EME)?

Electro membrane extraction, or EME, is a miniaturized liquid-liquid extraction technique developed for sample preparation of aqueous samples prior to analysis by chromatography, electrophoresis, mass spectrometry, and related techniques in analytical chemistry.

Why Electro Membrane Extraction (EME)

EME provides rapid extraction, efficient sample clean-up and high selectivity for ionizable compounds from blood samples, other body fluids and environmental samples. Under optimized conditions, EME can provide pure samples for mass spectrometry directly, without need for chromatography.

Excellent reproducibility

With EME’s excellent reproducibility you move reproducibility issues from the sample preparation to the MS.

Pure extracts

An additional advantage of Electro Membrane Extraction is that this sample preparation method is non-destructive i.e. you could re-extract your sample(s) whenever needed.

“Green” sample preparation method

“Green” sample preparation method due to low power consumption and almost no need for organic solvents.

How does EME work?

Two separate vials holding the sample solution and the acceptor solution.

The vials are separated by an organic solvent immobilized in the pores of a polymeric membrane, called the supported liquid membrane (SLM).

By applying an electric field across the membrane, charged analytes are transported by electrokinetic migration from the sample solution into the acceptor solution, while non-ionic analytes and ions of opposite charge are withheld in the sample solution.

You now have pure sample extract. Under optimized conditions, EME can provide very clean extracts with reduced matrix effects i.e. a complete discrimination of proteins and phospholipids, reducing down-time of your MS instruments. The extracts can be injected directly on LC-MS instruments using very short LC-methods before MS.

World’s first commercial EME instrument

ETN has developed the first commercial EME instrument. Please contact us to purchase our first EME instrument (ETN-1 EME) or pre-order our new 12 position instrument (ETN-12 EME).

Standardizing EME

We’ve standardized the EME process, making it perfect for R&D and application development.

Complete extraction kit

For an easy ETN-12 EME use, we provide you with an extraction kit containing: vials, membranes, unions and vial holder.

Environmentally friendly

“Green” sample preparation method due to low power consumption and almost no need for organic solvents.

Fast and selective

EME offers fast and selective sample extraction of ionizable compounds from your complex matrices.

Multi sample capable

12 samples can be extracted in parallel. In future devices, it will also be possible to use 96-well plates.

Single step sample preparation

With ETN-12 EME, your sample preparation will require only one single step to achieve pure extracts ready for analysis.

Extraction Kit

The ETN start kit includes everything you need to get the EME device up and running*:

Conductive vials (ETN 201002-1)
Membranes (ETN 201001-3 )
Union (ETN 201001-2)
Transparent buckets to make the vial fit into the auto sampler

* ETN do not sell solvents. Please contact our partners if you need to buy specific solvents.

About ETN

Extraction Technologies Norway AS was established in 2013 with a mission to develop a completely different, novel and innovative sample preparation method, which should be groundbreaking in terms of simplicity, speed of method and purity of samples.

For this purpose and based on our patented technology, we have developed the world’s first commercial instrument that uses electro-membrane extraction to prepare samples for analysis.

About ETN

Our distributors

Germany & Benelux: Dichrom

For almost two decades, Dichrom has been an established supplier in the liquid chromatography sector, offering a comprehensive range of products and expertise for HPLC and UHPLC as well as accompanying technologies. To this day, dichrom remains focused on liquid chromatography, with a portfolio of columns, accessories as well as sample preparation equipment that cover all routine and specialized requirements in modern chromatographic analysis.

Website: https://dichrom.com

Norway: Teknolab

Teknolab has been a part of the Norwegian chemical ecosystem since 2000 and represent several of the world‘s leading manufacturers of analysis equipment. Teknolab is Norway‘s largest supplier of HPLC columns, pumps for dust and solvent measurements, inorganic standards, as well as all kinds of tubing/pipes in narrow dimensions.

Website: https://teknolab.no

Sweden: Teknolab Sorbent

Teknolab Sorbent is a strong player in Sweden‘s chemical world.
Chemical analysis is something of a detective work. The result of your work should usually be as accurate as possible and as reliable as possible. Even if you adapt the accuracy to the specific area of use, it is important that the precision of the measurement is known - and this is where we at Teknolab come into the picture.

Website: https://teknolab.se

Rest of the world

If you are distributor or potential customer outside the markets above, please contact ETN directly for more information. Either on post@etn-eme.com or directly through our website: https://etn-eme.com