Application note for Atomoxetine
Created 03.04.2025 14:18
About ETN
Extraction Technologies Norway AS was established in 2013 with a mission to develop a completely different, novel and innovative sample preparation method, which should be groundbreaking in terms of simplicity, speed of method and purity of samples. For this purpose and based on our patented technology, we have developed the world’s first commercial instrument that uses electro-membrane extraction to prepare samples for analysis. With this EME technology charged molecules can be extracted from difficult matrices like e.g. blood or urine in an unprecedented purity, ready for direct analysis.
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✅Atomoxetine can be ionized and is therefore suitable for EME.
About Atomoxetine
Atomoxetine is a secondary amino compound having methyl and 3-(2-methylphenoxy)-3-phenylpropan-1-yl substituents. It has a role as an adrenergic uptake inhibitor, an antidepressant, a xenobiotic and an environmental contaminant. It is an aromatic ether, a secondary amino compound and a member of toluenes.
Analyte information
| Molecular formula | C17H21NO |
| Isomeric SMILES | CC1=CC=CC=C1O[C@H](CCNC)C2=CC=CC=C2 |
| LogP | 3.81 |
| Molecular weight | 255.35 |
| CID | 54841 |
| URL to analyte information source | Link |
Application note
Consumables used
| Vial donor (product number) | 201002-1 |
| Vial Acceptor (product number) | 201002-1 |
| Membrane (product number) | 201001-3 |
Extraction parameters
| Matrix | Serum |
| Voltage (v) | 150 |
| RPM | 875 |
| Extraction time (min) | 30 |
| Membrane type | PP2E |
| Membrane solvent | NPOE |
| Amount of membrane solvent (μL) | 9 |
| Donor vial size | 600 |
| Donor solution | Aliquots of serum (100 μL, spiked or analyte free), internal standard solution (25 μL) and 0.1% formic acid (v/v, 175 μL) |
| Donor amount (μL) | 300 |
| Acceptor vial size (μL) | 600 |
| Acceptor solution | 0.1% formic acid |
| Acceptor amount (μL) | 300 |
| Analyte category | Psyoactive drug |
Method performance
| Linear range | 40-4000 |
| Concentration unit | nM |
| Recovery (%) | 96 |
| R2 | 0.9995 |
| Intra day precision (%) | 3 |
| Inter-day precision (%) | 3 |
| Accuracy (%) | −1,3 |
| Matrix effects (%) | 101 |
| Analytical method | UHPLC-MS/MS |
| Publication URL | Link |
Analytical method
| LC instrument | Acquity UPLC® I-Class |
| Column | Acquity UPLC® BEH C18 (2.1 × 50 mm, 1.7 µm particles) |
| Injection volume (µL) | 0,4 |
| Column temperature (C) | 50 |
| Flow rate | 0.6 mL/min |
| Mobile phase | A: 5 mM ammonium formate with pH 10.1. B: methanol |
| LC-gradient | 0.0-0.9 min: 60-90%B, 98% at 1.9 min, 60% at 3.0 min. |
| MS instrument | Waters Xevo TQ-S tandem mass spectrometer |
| Internal standard | Atomoxetine-d3 |
| MRM transitions | 256 > 44, 256 > 148 |
| Capillary voltage | 1 kV |
| Ion source temperature | 120 |
| Drying/desolvation gas | 1000 L/h @ 600C |
| Cone gas flow | 150 L/h |
What is Electromembrane Extraction (EME)?
Electro membrane extraction, or EME, is a miniaturized liquid-liquid extraction technique developed for sample preparation of aqueous samples prior to analysis by chromatography, electrophoresis, mass spectrometry, and related techniques in analytical chemistry.
Why Electro Membrane Extraction (EME)
EME provides rapid extraction, efficient sample clean-up and high selectivity for ionizable compounds from blood samples, other body fluids and environmental samples. Under optimized conditions, EME can provide pure samples for mass spectrometry directly, without need for chromatography.
Excellent reproducibility
With EME’s excellent reproducibility you move reproducibility issues from the sample preparation to the MS.
Pure extracts
An additional advantage of Electro Membrane Extraction is that this sample preparation method is non-destructive i.e. you could re-extract your sample(s) whenever needed.
“Green” sample preparation method
“Green” sample preparation method due to low power consumption and almost no need for organic solvents.
How does EME work?
World’s first commercial EME instrument
ETN has developed the first commercial EME instrument. Please contact us to purchase our first EME instrument (ETN-1 EME) or pre-order our new 12 position instrument (ETN-12 EME).
Standardizing EME
We’ve standardized the EME process, making it perfect for R&D and application development.
Complete extraction kit
For an easy ETN-12 EME use, we provide you with an extraction kit containing: vials, membranes, unions and vial holder.
Environmentally friendly
“Green” sample preparation method due to low power consumption and almost no need for organic solvents.
Fast and selective
EME offers fast and selective sample extraction of ionizable compounds from your complex matrices.
Multi sample capable
12 samples can be extracted in parallel. In future devices, it will also be possible to use 96-well plates.
Single step sample preparation
With ETN-12 EME, your sample preparation will require only one single step to achieve pure extracts ready for analysis.
Extraction Kit
The ETN start kit includes everything you need to get the EME device up and running*:
- Conductive vials (ETN 201002-1)
- Membranes (ETN 201001-3 )
- Union (ETN 201001-2)
- Transparent buckets to make the vial fit into the auto sampler
About ETN
Extraction Technologies Norway AS was established in 2013 with a mission to develop a completely different, novel and innovative sample preparation method, which should be groundbreaking in terms of simplicity, speed of method and purity of samples.
For this purpose and based on our patented technology, we have developed the world’s first commercial instrument that uses electro-membrane extraction to prepare samples for analysis.
About ETN
Extraction Technologies Norway AS was established in 2013 with a mission to develop a completely different, novel and innovative sample preparation method, which should be groundbreaking in terms of simplicity, speed of method and purity of samples.
For this purpose and based on our patented technology, we have developed the world’s first commercial instrument that uses electro-membrane extraction to prepare samples for analysis.
Our distributors
Germany & Benelux: Dichrom
For almost two decades, Dichrom has been an established supplier in the liquid chromatography sector, offering a comprehensive range of products and expertise for HPLC and UHPLC as well as accompanying technologies. To this day, dichrom remains focused on liquid chromatography, with a portfolio of columns, accessories as well as sample preparation equipment that cover all routine and specialized requirements in modern chromatographic analysis.
Website: https://dichrom.com
Norway: Teknolab
Teknolab has been a part of the Norwegian chemical ecosystem since 2000 and represent several of the world‘s leading manufacturers of analysis equipment. Teknolab is Norway‘s largest supplier of HPLC columns, pumps for dust and solvent measurements, inorganic standards, as well as all kinds of tubing/pipes in narrow dimensions.
Website: https://teknolab.no
Sweden: Teknolab Sorbent
Teknolab Sorbent is a strong player in Sweden‘s chemical world.
Chemical analysis is something of a detective work. The result of your work should usually be as accurate as possible and as reliable as possible. Even if you adapt the accuracy to the specific area of use, it is important that the precision of the measurement is known - and this is where we at Teknolab come into the picture.
Website: https://teknolab.se
Rest of the world
If you are distributor or potential customer outside the markets above, please contact ETN directly for more information. Either on post@etn-eme.com or directly through our website: https://etn-eme.com